mmi CellCut Plus - Features

Choose mmi CellCut Plus in order to benefit from a range of advantages that will simplify your work more effectively. These includes:

• Contamination-free microdissection, attributed to the patented mmi IsolationCap technology
• Easy operation with high level automation
• Outstanding image quality
• High-precision laser cutting < 1µm/40x
• Long laser lifespan
• Safe, reliable operation
• Only MMI offers positive sample inspection, a 100% sure way of seeing what you have isolated
• Live cell microdissection
• Modular, upgradeable instruments from MMI such as the mmi CellManipulator Plus or the mmi CellEctor Plus
• mmi CellCut Plus is compatible with Olympus IX71 and IX81, and inverted Nikon microscopes including the new Nikon Ti
  

The new mmi CellCut Plus also comes with the following extra features:  

• New single- or multi mmi CapLift designed as a plug and play module with adjustable cap contact pressure
• New digital camera series mmi CellCamera: - Ultra high sensitive with progressive scan - high quality imaging and contrast - b/w version for high-end fluorescence
• New mmi CellTools software - advanced collection method - lens offset
• Predefined Target Positioning (PTP) - Enables individual positioning of the microdissected and isolated targets within the isolation cap, helping in verifying the quality and quantity of the extracted material
• The patented serial section function allows the marking of target regions onto one or more unstained sections by using a stained top section as a template. Differences in shape, angle and position are automatically compensated by the software giving highly accurate results. This saves time and money by reducing reagent usage and limits the RNA degradation.

• The Z-Drill function allows the cutting of wet and thick tissue without the need for increased laser power. The laser is refocused through the sample and so facilitates cutting in a spiral. Providing a smooth, clear cut without the need of a high powered laser the result is superior cutting with minimised tissue damage.

The mmi CellCut Plus enables contamination-free isolation of cells in living culture with the new mmi LiveCell Chamber. The new mmi LiveCell Chamber consists of a membrane ring, a petri dish, and the microdissection chamber.

• Adherent cells are grown in a special metal ring on a membrane inside a petri dish.
• Once a desired cell density is reached, the membrane ring is transferred to the microdissection chamber.
• The cell or cells of interest are selected and cut using laser microdissection.
• In addition, unwanted cells can be destroyed by using a single laser shot.
• The membrane ring is then removed and only the cells of interest remain in the microdissection chamber.
• This process can be repeated using the same metal ring in a new microdissection chamber to select and separate more than one cell type.
• Once isolation is complete, more media is added to the microdissection chamber and the cells can be re-cultivated.

The isolation of single cells is a unique and highly useful tool in wide range of research and clinical applications, including individual cell analysis, clonal expansion, and stem cell research. Enzyme treatments (i.e. trypsin), other potentially harmful selective reagents, as well as time consuming repetitive enrichments can be avoided. The benefit is an increase in reliability, effectiveness, and accuracy of your research.

View Application Workflow

Customize mmi CellCut Plus with a choice of value-added optional features for an even easier, more automated operation such as:

• High power solid state laser for special applications on plants and other special tissues
• mmi CellExplorer colour recognition software which automatically recognizes cells under fluorescence and in bright field
• mmi MultiCap uses eight mmi Isolation-Caps which can be attached simultaneously, facilitating automatic switching from one cap onto the other during group cutting
• MultiSlide functionality allows the simulta-neous microdissection of up to three mmi MembraneSlides
• Touch screen pen for more comfort